Identification of Plant Enhancers and Their Constituent Elements by STARR-seq in Tobacco Leaves.
Jores T, Tonnies J, Dorrity MW, Cuperus J, Fields S, Queitsch C
Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155. PubMed Article
Jores T, Tonnies J, Dorrity MW, Cuperus J, Fields S, Queitsch C
Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149416 | pPSup | Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. |
| 149417 | pPSdown | Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene. |
| 149418 | pPSup2 | Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter. |
| 149419 | pPSdown2 | Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene. |
| 149420 | pPSup_iGFP | Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron. |
| 149421 | pPSint | Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene. |
| 149422 | pPSup_35Senh(fwd)_35Spr+synJ | Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer. |
| 149423 | pZS*11_4enh | Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9). |