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Temperature-Inducible Precision-Guided Sterile Insect Technique.
Kandul NP, Liu J, Akbari OS
CRISPR J. 2021 Dec;4(6):822-835. doi: 10.1089/crispr.2021.0077. Epub 2021 Nov 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
1494241137=Hsp70Bb-Cas9[dsRed]The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)
1494251046E=dgRNAs[traB,bTub]The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.
1494261098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.
1494271098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.
1532841137B=Hsp70Bb-Cas9The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

Antibodies from Article