A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli.
Jervis AJ, Hanko EKR, Dunstan MS, Robinson CJ, Takano E, Scrutton NS
Microb Biotechnol. 2021 Mar 12. doi: 10.1111/1751-7915.13780. PubMed Article
Jervis AJ, Hanko EKR, Dunstan MS, Robinson CJ, Takano E, Scrutton NS
Microb Biotechnol. 2021 Mar 12. doi: 10.1111/1751-7915.13780. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153034 | pSIMcpf1 | For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1. |
| 153035 | pTF-ahpC-rfp | For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene. |
| 153036 | pTF-lacZ | For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA. |
| 153037 | pTF-lacZ-rfp | CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene. |
| 153038 | pBbS8c-ddcpf1-rfp | For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene. |
| 153039 | pBbS8c-ddcpf1-Δ | For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer. |