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De Novo Engineering of Corynebacterium glutamicum for l-Proline Production.
Zhang J, Qian F, Dong F, Wang Q, Yang J, Jiang Y, Yang S
ACS Synth Biol. 2020 Jul 17;9(7):1897-1906. doi: 10.1021/acssynbio.0c00249. Epub 2020 Jul 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
153337pCgCas9_recTDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
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153338pCgsgRNA_crtYfDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
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