A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases.
Pullmann P, Knorrscheidt A, Munch J, Palme PR, Hoehenwarter W, Marillonnet S, Alcalde M, Westermann B, Weissenborn MJ
Commun Biol. 2021 May 12;4(1):562. doi: 10.1038/s42003-021-02076-3. PubMed Article
Pullmann P, Knorrscheidt A, Munch J, Palme PR, Hoehenwarter W, Marillonnet S, Alcalde M, Westermann B, Weissenborn MJ
Commun Biol. 2021 May 12;4(1):562. doi: 10.1038/s42003-021-02076-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153487 | pAGT572_Nemo | Level 1 episomal expression plasmid for S. cerevisiae |
| 153488 | pPAP001 | Level 1 episomal expression plasmid for P. pastoris |
| 153489 | pPAP002 | Level 1 episomal expression plasmid for P. pastoris |
| 153490 | pPAP003 | Level 2 integrative plasmid for stable expression in P. pastoris |
| 153491 | pAGM9121_Sce-Prepro SP | S. cerevisiae Prepro Signal Peptide Donor Plasmid |
| 153492 | pAGM9121_Kma-Inulinase SP | Kluyveromyces marxianus Inulinase Signal Peptide Donor Plasmid |
| 153493 | pAGM9121_Sce-Invertase 2 SP | S. cerevisiae Invertase Signal Peptide Donor Plasmid |
| 153494 | pAGM9121_Sce-Acid Phosphatase SP | S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid |
| 153495 | pAGM9121_Gma-UPO SP | Galerina marginata Unspecific Peroxygenase Signal Peptide Donor Plasmid |
| 153496 | pAGM9121_Hsa-Serum Albumin SP | Human Serum Albumin Signal Peptide Donor Plasmid |
| 153497 | pAGM9121_Aaw-Glucoamylase SP | Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid |
| 153498 | pAGM9121_Sce-Killer Protein SP | S. cerevisiae Killer Signal Peptide Donor Plasmid |
| 153499 | pAGM9121_Mro-UPO SP | Marasmius rotula Unspecific Peroxygenase Signal Peptide Donor Plasmid |
| 153500 | pAGM9121_Cfo-CPO SP | Caldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor Plasmid |
| 153501 | pAGM9121_Cgl-UPO SP | Chaetomium globosum Unspecific Peroxygenase Signal Peptide Donor Plasmid |
| 153502 | pAGM9121_Cci-UPO SP | Coprinopsis cinerea Unspecific Peroxygenase Signal Peptide Donor Plasmid |
| 153503 | pAGM9121_Ani- α Amylase SP | Aspergillus niger α Amylase Signal Peptide Donor Plasmid |
| 153504 | pAGM9121_Sce- α Galactosidase SP | α Galactosidase Signal Peptide Donor Plasmid |
| 153505 | pAGM9121_Gga-Lysozym SP | Chicken Lysozym Signal Peptide Donor Plasmid |
| 153506 | pAGM9121_Aae-UPO* SP | Agrocybe aegerita Unspecific peroxygenase (UPO*) Signal Peptide Donor Plasmid |
| 153507 | pAGM9121_Mth-UPO SP | Unspecific peroxygenase Signal Peptide Donor Plasmid |
| 153508 | pAGM9121_GFP11 | GFP11 C-terminal Tag Donor Plasmid |
| 153509 | pAGM9121_OctaHis-GFP11 | OctaHis-GFP11 C-terminal Tag Donor Plasmid |
| 153510 | pAGM9121_TEV-His-GFP11 | TEV-His-GFP11 C-terminal Tag Donor Plasmid |
| 153511 | pAGM9121_His2-GFP11 | His2-GFP11 C-terminal Tag Donor Plasmid |
| 153512 | pAGM9121_Strep II | Strep II C-terminal Tag Donor Plasmid |
| 153513 | pAGM9121_TwinStrep | TwinStrep C-terminal Tag Donor Plasmid |
| 153514 | pAGM9121_TwinStrep-GFP11 | TwinStrep-GFP11 C-terminal Tag Donor Plasmid |
| 153515 | pAGM22082_sfGFP1-10 | E.coli expression plasmid for the production of GFP1-10 |