Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm Genetic Requirements in Enterococcus faecalis.

Afonina I, Ong J, Chua J, Lu T, Kline KA
mBio. 2020 Oct 20;11(5):e01101-20. doi: 10.1128/mBio.01101-20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
153516	pMSP3545-dCas9Str	Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone
153517	pGCP123-EbpA_g1	sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded
153518	pGCP123-GFP_g1	sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded