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Scott McComb Lab: Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of large genetic payloads for rapid cell engineering
Unpublished

Plasmids from Article

ID Plasmid Purpose  
154090pQCi-mB2m-sgRNApQCi backbone with sgRNA targeting murine Beta-2-microglobulin
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154091pSCIP-mB2MSelf-Cutting and Integrating CRISPR backbone targeting murine B2M
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154093pQCi-hTRAC-sgRNApQCi backbone with sgRNA targeting human TCR-alpha common chain
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154094pSCIP-hTRACSelf-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
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154095pSCIP-hCD69-TdTomatoSelf-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene
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154096pSCIPpay-BFP reporterSelf-Cutting and Integrating Payload CRISPR backbone with BFP reporter
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154345pQCiSelf-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)
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