pSCIP-hTRAC
(Plasmid #154094)

• Purpose: Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
• Depositing Lab: Scott McComb
• Publication: Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of large genetic payloads for rapid cell engineering (unpublished)

Backbone

• Vector backbone: pQCi
  (Search Vector Database)
• Backbone manufacturer: Derived from pX458 (Zhang lab)
• Backbone size w/o insert (bp): 9500
• Modifications to backbone: Modular BAIT site integrated (BsaI sites) Intron integrated into spCas9 sequence
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: GFP and tdTomato
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgRNA and BAIT targeting human TRAC locus
• Alt name: TCTCTCAGCTGGTACACGGC
• Species: H. sapiens (human)

Resource Information

• Supplemental Documents:
  • pSCIP-hTRAC-1a.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry