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Imd pathway-specific immune assays reveal NF-kappaB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells.
Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524. PubMed Article

Plasmids from Article

ID Plasmid Purpose
154152pKM45Firefly luciferase with no promoter for negative control for luciferase assays
154153pKM44Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter
154154pKM51Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site
154155pKM52Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site
154156pKM53Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel1A NF-κB site
154157pKM54Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site
154158pKM59Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.
154159pKM60Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154160pKM62Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154161pAA1Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154162pAA2Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154163pAA3Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154164pKM7mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
154165pKM28Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus
154166pKM5Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
154167pKM33Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
154168pKM3Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Antibodies from Article