RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.
Rheinemann L, Downhour DM, Bredbenner K, Mercenne G, Davenport KA, Schmitt PT, Necessary CR, McCullough J, Schmitt AP, Simon SM, Sundquist WI, Elde NC
Cell. 2021 Sep 27. pii: S0092-8674(21)01055-2. doi: 10.1016/j.cell.2021.09.008. PubMed Article
Rheinemann L, Downhour DM, Bredbenner K, Mercenne G, Davenport KA, Schmitt PT, Necessary CR, McCullough J, Schmitt AP, Simon SM, Sundquist WI, Elde NC
Cell. 2021 Sep 27. pii: S0092-8674(21)01055-2. doi: 10.1016/j.cell.2021.09.008. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154169 | pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA | expresses squirrel monkey CHMP3 in mammalian cells |
| 154170 | pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA | expresses squirrel monkey CHMP3 in mammalian cells |
| 154171 | pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA | expresses squirrel monkey CHMP3 in mammalian cells |
| 154172 | pEF1α-squirrel-monkey-full-length-CHMP3-HA | expresses squirrel monkey CHMP3 in mammalian cells |
| 154173 | pEF1α-squirrel-monkey-CHMP3(155)-HA | expresses squirrel monkey CHMP3(155) in mammalian cells |
| 154174 | pEF1α-squirrel-monkey-retroCHMP3-HA | expresses squirrel monkey retroCHMP3 in mammalian cells |
| 154175 | pCMV(WT)-mouse-full-length-CHMP3-HA | expresses mouse CHMP3 in mammalian cells |
| 154176 | pEF1α-mouse-full-length-CHMP3-HA | expresses mouse CHMP3 in mammalian cells |
| 154177 | pEF1α-mouse-CHMP3(147)-HA | expresses mouse CHMP3(147) in mammalian cells |
| 154178 | pEF1α-mouse-retroCHMP3-HA | expresses mouse retroCHMP3 in mammalian cells |
| 154179 | pEF1α-spider-monkey-retroCHMP3-HA | expresses spider monkey retroCHMP3 in mammalian cells |
| 154180 | pCAG-OSF-PP-human-CHMP3 | expresses human CHMP3 in mammalian cells |
| 154181 | pCAG-OSF-PP-human-CHMP3(150) | expresses human CHMP3(150) in mammalian cells |
| 154182 | pCAG-OSF-PP-squirrel-monkey-CHMP3 | expresses squirrel monkey CHMP3 in mammalian cells |
| 154183 | pCAG-OSF-PP-squirrel-monkey-CHMP3(155) | expresses squirrel monkey CHMP3(155) in mammalian cells |
| 154184 | pCAG-OSF-PP-squirrel-monkey-retroCHMP3 | expresses squirrel monkey retroCHMP3 in mammalian cells |
| 154185 | pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP | expresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells |
| 154247 | pCAG-OSF-PP | backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag |