HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.
Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, Kempa S, Huttelmaier S, Selbach M, Landthaler M
Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7. PubMed Article
Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, Kempa S, Huttelmaier S, Selbach M, Landthaler M
Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154448 | pDONR221_HDLBP | For recombination of HDLBP |
| 154450 | pFRT/TO/FLAG/HA-HDLBP | Expression of FLAG/HA-tagged HDLBP |
| 154453 | pcDNA5-BirA-FLAG-N-term-HDLBP | Expression of N-term BirA tagged HDLBP |
| 154454 | pFRTpsiCHECK | Expression of renilla luciferase and firefly luciferase |
| 154459 | pFRTpsiCHECK_Gaussia_luciferase | Expression of gaussia luciferase and firefly luciferase |
| 154461 | pFRTpsiCHECK_SEAP | Expression of SEAP and firefly luciferase |