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Expanding the Chemogenetic Toolbox by Circular Permutation.
Lee YT, He L, Zhou Y
J Mol Biol. 2020 May 1;432(10):3127-3136. doi: 10.1016/j.jmb.2020.03.033. Epub 2020 Apr 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
155389mCh-cpFRB1Express cpFRB1 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2
159645mCh-cpFRB2Express cpFRB2 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2
165026GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP
165028GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP
165029mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry
165030mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Antibodies from Article