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A viral toolkit for recording transcription factor-DNA interactions in live mouse tissues.
Cammack AJ, Moudgil A, Chen J, Vasek MJ, Shabsovich M, McCullough K, Yen A, Lagunas T, Maloney SE, He J, Chen X, Hooda M, Wilkinson MN, Miller TM, Mitra RD, Dougherty JD
Proc Natl Acad Sci U S A. 2020 May 5;117(18):10003-10014. doi: 10.1073/pnas.1918241117. Epub 2020 Apr 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86949pAAV-short Cre-on G/WGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV
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86950AAV-short_BrokenHeart (no BC)AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
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86954pAAV_GWRev_DestGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)
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86955pAAV_GW_DestGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.
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154889AAV-PB-SRT-tdTomatopiggyBac self-reporting transposon with tdTomato marker in AAV backbone
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Antibodies from Article