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Ten catalytic snapshots of rhomboid intramembrane proteolysis from gate opening to peptide release.
Cho S, Baker RP, Ji M, Urban S
Nat Struct Mol Biol. 2019 Oct;26(10):910-918. doi: 10.1038/s41594-019-0296-9. Epub 2019 Sep 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
155131pET15-His-∆NGlpGExpresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b
155132pET15-His-∆NGlpG(Y205F)Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b
155133pET15-His-∆NGlpG(C104A/M247C)Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b
155134pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b
155135pGEX-HA-GlpGExpresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1
155136pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1

Antibodies from Article