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A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo.
Moore CL, Papa LJ 3rd, Shoulders MD
J Am Chem Soc. 2018 Sep 19;140(37):11560-11564. doi: 10.1021/jacs.8b04001. Epub 2018 Jul 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
156454T7 promoter + filler DNA reporter plasmidA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.
156455T7 promoter + rpsL reporter plasmidA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.
156456T7 promoter + terminators reporter plasmidA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
156457Dual opposing T7 promoters reporter plasmidA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.
156459rApoIAn E. coli strain that expresses rat APOBEC1. Used as a control strain for mutagenesis experiments with MutaT7.
156460MutaT7An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.
156461drApo1–T7An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.

Antibodies from Article