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Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering
Priode JH, Haynes KA
Protocols.io, Jan 19, 2021
Article

Plasmids from Article

ID Plasmid Purpose  
157649GGDest1-AmpGolden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert
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157650GGDest2-AmpGolden Gate assembly of a pET28-compatible fusion-protein ORF insert
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157651GGDest3-AmpGolden Gate assembly of a pTXB1 compatible fusion-protein ORF insert
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157652GGDestX1-AmpGolden Gate assembly and cell-free (bacterial) expression of fusion-proteins
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157654GGDestX2-AmpGolden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA
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