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One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening.
Francino-Urdaniz IM, Steiner PJ, Kirby MB, Zhao F, Haas CM, Barman S, Rhodes ER, Leonard AC, Peng L, Sprenger KG, Jardine JG, Whitehead TA
Cell Rep. 2021 Aug 31;36(9):109627. doi: 10.1016/j.celrep.2021.109627. Epub 2021 Aug 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
157971SARS-CoV-2 Spike (S) Ectodomain Library AEncodes Spike ectodomain mutations that stabilize 'up' prefusion conformation
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157972SARS-CoV-2 Spike (S) Ectodomain Library BEncodes Spike ectodomain mutations that stabilize 'up' prefusion conformation
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157973SARS-CoV-2 Spike (S) Ectodomain Library CEncodes Spike ectodomain mutations that stabilize 'up' prefusion conformation
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168776SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations
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168777SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations
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168778pJS697Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination
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168779pJS699Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination
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174293SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 1 (positions 333-436)This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.
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174294SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 2 (positions 437-527)This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.
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174295SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 1 (positions 333-436)This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.
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174296SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 2 (positions 437-527)This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.
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174298pIFU001Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
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174299pIFU002Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
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Recombinant Antibodies from Article