Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.
Wang G, Zhao Z, Ke J, Engel Y, Shi YM, Robinson D, Bingol K, Zhang Z, Bowen B, Louie K, Wang B, Evans R, Miyamoto Y, Cheng K, Kosina S, De Raad M, Silva L, Luhrs A, Lubbe A, Hoyt DW, Francavilla C, Otani H, Deutsch S, Washton NM, Rubin EM, Mouncey NJ, Visel A, Northen T, Cheng JF, Bode HB, Yoshikuni Y
Nat Microbiol. 2019 Dec;4(12):2498-2510. doi: 10.1038/s41564-019-0573-8. Epub 2019 Oct 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
158207pW172lox landing pad plasmid with mariner transposon and transposase used for CRAGE
158210pW34LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.
158211pW5Y_AprBAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

Antibodies from Article