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Real-time monitoring of peroxiredoxin oligomerization dynamics in living cells.
Pastor-Flores D, Talwar D, Pedre B, Dick TP
Proc Natl Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 10.1073/pnas.1915275117. PubMed Article

Plasmids from Article

ID Plasmid Purpose
158224pcDNA3.1(−) mCER-Prx2Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)
158225pLPCX mCER-Prx2Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)
158226pET-SUMO mCER-Prx2Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)
158227pLPCX mCER-Prx1Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)
158228pLPCX roGFP2-Prx2(C172A)Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)
169230pLPCX roGFP2-Prx2Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation)

Antibodies from Article