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A confinable home and rescue gene drive for population modification.
Kandul NP, Liu J, Bennett JB, Marshall JM, Akbari OS
Elife. 2021 Mar 5;10. pii: 65939. doi: 10.7554/eLife.65939. PubMed Article

Plasmids from Article

ID Plasmid Purpose
159671874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.
159672874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.
1596731095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.
1596741026L=U6-3-gRNA#1[Pol-γ35]Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.
1596751026M=U6-3-gRNA#2[Pol-γ35]Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.
1596761073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
1596771073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

Antibodies from Article