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Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior.
Goswami D, Chen, Yang Y, Gudla PR, Columbus J, Worthy K, Rigby M, Wheeler M, Mukhopadhyay S, Powell K, Burgan W, Wall V, Esposito D, Simanshu DK, Lightstone FC, Nissley DV, McCormick F, Turbyville T
Elife. 2020 Jan 20;9. pii: 47654. doi: 10.7554/eLife.47654.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159678pDest-303Gateway destination vector for mammalian protein expression
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159679R703-M72-303: CMV51p> Halotag-Hs.KRAS4bMammalian protein expression of KRAS4b-Halotag fusion
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159680R713-M85-303: CMV51p> Halotag-Hs.KRAS4aMammalian protein expression of KRAS4a-Halotag fusion
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159681R713-M88-303: CMV51p> Halotag-Hs.HRASMammalian protein expression of HRAS-Halotag fusion
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159682R713-M91-303: CMV51p> Halotag-Hs.NRASMammalian protein expression of NRAS-Halotag fusion
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159683R703-M80-303: CMV51p> Halotag-Kras4bHVRMammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion
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159684R713-M48-303: CMV51p> Halotag-Kras4aHVRMammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion
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159685R713-M50-303: CMV51p> Halotag-HRasHVRMammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion
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159686R713-M52-303: CMV51p> Halotag-NRasHVRMammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion
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Antibodies from Article