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Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane.
Lakshman B, Messing S, Schmid EM, Clogston JD, Gillette WK, Esposito D, Kessing B, Fletcher DA, Nissley DV, McCormick F, Stephen AG, Jean-Francois FL
J Biol Chem. 2019 Feb 8;294(6):2193-2207. doi: 10.1074/jbc.RA118.005669. Epub 2018 Dec 17. PubMed Article

Plasmids from Article

ID Plasmid Purpose
159696R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)
159697R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)
159698R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)
159699R725-X11-566: His6-MBP-tev-Hs.LGALS3E. coli protein expression of His6-MBP-tev-Hs.LGALS3
159700R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3E. coli protein expression

Antibodies from Article