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RNA timestamps identify the age of single molecules in RNA sequencing.
Rodriques SG, Chen LM, Liu S, Zhong ED, Scherrer JR, Boyden ES, Chen F
Nat Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 10.1038/s41587-020-0704-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159922pAAV-Ef1a-MCP-huADARE488QT490AFusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5
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159923pcDNA3.1-GAVPOGAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133
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159924pTRE3G-iRFP-B1-timestamp_ATimestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.
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159925pLenti-5xUASG-iRFP-B3-timestamp-Atimestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.
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159926pTRE3G-c-fos-iRFP-B3-repRNA-ATimestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.
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Antibodies from Article