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Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products.
Rajkumar AS, Morrissey JP
Microb Cell Fact. 2020 Nov 11;19(1):207. doi: 10.1186/s12934-020-01461-7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
160004pMTU-DO-URALow-copy expression vector for K. marxianus, uracil selection
160005pMTU-DO-HISLow-copy expression vector for K. marxianus, histidine selection
160006pMTU-DO-LEULow-copy expression vector for K. marxianus, leucine selection
160007pMTU-DO-G418Low-copy expression vector for K. marxianus, G418 selection
160008pMTU-DO-HPHLow-copy expression vector for K. marxianus, hygromycin selection
160009pI1-MTU-DO-URAIntegrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.
160012pI1-MTU-DO-G418Integrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.
160013pI1-MTU-DO-LEUIntegrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.
160014pI1-MTU-DO-HPHIntegrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.
160015pI2-MTU-DO-URAIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.
160016pI2-MTU-DO-HISIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.
160017pI2-MTU-DO-LEUIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.
160018pI2-MTU-DO-G418Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.
160019pI2-MTU-DO-HPHIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.
160020pI3-MTU-DO-URAIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.
160021pI3-MTU-DO-HISIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.
160023pI3-MTU-DO-G418Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.
160025pI4-MTU-DO-URAIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.
160026pI4-MTU-DO-HISIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.
160029pI4-MTU-DO-LEUIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.
160030pI6-MTU-DO-URAIntegrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.
160142pI1-MTU-DO-HISIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.
160176pI3-MTU-DO-LEUIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.
160207pI3-MTU-DO-HPHIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.
160215pI4-MTU-DO-G418Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.
160216pI4-MTU-DO-HPHIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection
160339pMTU-DO-URA-C4High-copy expression vector for K. marxianus, uracil selection
160340pMTU-DO-HIS-C4High-copy expression vector for K. marxianus, histidine selection
160341pMTU-DO-LEU-C4High-copy expression vector for K. marxianus, leucine selection
160342pMTU-DO-G418-C4High-copy expression vector for K. marxianus, G418 selection
160343pMTU-DO-HPH-C4High-copy expression vector for K. marxianus, hygromycin selection

Antibodies from Article