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Addgene
Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.
Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI
Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779. PubMed Article

Plasmids from Article

ID Plasmid Purpose
160050pCA528 AMSH 1-178Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.
160051pCAG-OSF-KATNA1Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.
160052pCAG-OSF-KATNA1 R14AMammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.
160053pCAG-OSF-KATNA1 V55DMammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.
160054pCAG-Myc-KATNB1Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.
180609pCA528 AMSHLP 16-218Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83
180610pGEX-THTAP-CALPAIN-7 1-165Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129
180611pCA528 CALPAIN-7 residues 1-165Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25
180612pCA528 CALPAIN-7 residues 1-165, V18DBacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.
180613pCA528 CALPAIN-7 residues 1-165, F98DBacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.
180614pCA528 KATNA1 MIT residues 1-79Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.
180615pCA528 KATNA1 MIT residues 1-79, V55DBacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.
180618pCA528 KATNAL1 MIT residues 1-87Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.
180619pCA528 KATNAL2 residues 1-98Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.
180621pGEX-THTAP NRBF2 MIT residues 1-111Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.
180622pCA528 RPS6KC1 MIT, residues 235-316Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.
180623pCA528 RPS6KL1 MIT, residues 20-140Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.
180624pGEX-THTAP-SNX15, residues 263-342Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.
180625pCA528 SNX15 MIT residues 263-342Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14
180626pGEX-THTAP-SPARTIN, residues 1-101Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.
180627pCA528 SPASTIN , residues 112-196Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.
180628pCA528 SPASTIN , residues 108-200Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.
180629pCA528 SPASTIN , F124D, residues 108-200Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.
180634pCA528 SPASTIN , L177D, residues 108-200Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.
180636pCA528 USP8, residues 1-147Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.
180637pCA528 USP54, residues 731-878Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85
180638pET16B VPS4A, residues 1-84Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43
180639pGEX2T VPS4B, residues 1-86Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.
180640pGEX-THTAP-VPS9D1, residues 1-97Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.
180641pCA528 CHMP4B, residues 156-224, no CysBacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140
180642pCA528 CHMP4C, residues 156-233, no CysBacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.
180643pCA528 IST1, residues 315-360, no CysBacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.
180644pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.
180645pCAG-CHMP2A-myc, L216D/L219DMammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.
180646pLVX-mCherryLentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.
180647pLVX-mCherry-SPASTINLentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.
180648pLVX-mCherry-SPASTIN, F124DLentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.
180649pLVX-mCherry-SPASTIN, L177DLentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.
180650pLVX-mCherry-KATNA1Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.
180651pLVX-mCherry-KATNA1, V55DLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.
180652pLVX-CALPAIN-7-mCherryLentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.
180653pLVX-CALPAIN-7-mCherry, F98DLentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

Antibodies from Article