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An optimized toolbox for the optogenetic control of intracellular transport.
Nijenhuis W, van Grinsven MMP, Kapitein LC
J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
174621pB80-emptyEmpty backbone
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174622pB80-FKBP-mCherry-RAB11Rapalog-inducible coupling to recycling endosomes (RAB11) via FKBP
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174623pB80-iLID-mCherry-RAB11Optogenetic coupling to recycling endosomes (RAB11) via iLID
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174624pB80-iLID-mCherry-RAB5Optogenetic coupling to early endosomes (RAB5) via iLID
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174625pB80-LAMP1-mCherry-iLIDOptogenetic coupling to lysosomes (LAMP1) via iLID
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174626pB80-KIF1A(1-383)-GFP-SSPB(milli)Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)
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174627pB80-KIF1A(1-383)-GFP-SSPB(micro)Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
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174628pB80-KIF1A(1-383)-GFP-SSPB(nano)Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)
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174629pB80-KIF1A(1-365)-GFP-SSPB(micro)Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
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174630pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
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174631pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
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174632pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
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174633pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
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174634pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
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174635pB80-KIF1A(1-365)-mVenus-SSPB(micro)Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
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174636pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
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174637pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
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174638pB80-FRB-GFP-ppKin14VIb(861-1321)Rapalog-inducible coupling to dimeric moss kinesin-14 for retrograde transport via FRB
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174639pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
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174640pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
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174641pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
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174642pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
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174643pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
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174644pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes
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174645pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
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174646pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.
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174647pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
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174648HA-BICD2(1-500)-FRBRapalog-inducible coupling to dynein adaptor BICD2
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Recombinant Antibodies from Article