Structural basis for tuning activity and membrane specificity of bacterial cytolysins.

Shah NR, Voisin TB, Parsons ES, Boyd CM, Hoogenboom BW, Bubeck D
Nat Commun. 2020 Nov 16;11(1):5818. doi: 10.1038/s41467-020-19482-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
160513	pTricHis-ILY-WT-charge+	Expression of inserted ILY variant
160514	pTricHis-ILY-WT-no charge	Expression of inserted ILY variant
160515	pTricHis-ILY-WT-Y336W	Expression of inserted ILY variant
160516	pTricHis-ILY-WT-N338W	Expression of inserted ILY variant
160517	pTricHis-ILY-WT-L340W	Expression of inserted ILY variant
160518	pTricHis-ILY-WT-N342W	Expression of inserted ILY variant
160519	pTricHis-ILY-prepore	Expression of inserted ILY variant
160520	pTricHis-ILY-prepore-L340C	Expression of inserted ILY variant
160521	pTricHis-ILY-prepore-N342C	Expression of inserted ILY variant
160522	pTricHis-ILY-prepore-charge+	Expression of inserted ILY variant
160523	pTricHis-ILY-HB1lock	Expression of inserted ILY variant
160524	pTricHis-ILY-HB1lock-charge+	Expression of inserted ILY variant
160525	pTricHis-ILY-HB1lock-no charge	Expression of inserted ILY variant
160526	pTricHis-ILY-HB1lock-Y336W	Expression of inserted ILY variant
160527	pTricHis-ILY-HB1lock-N338W	Expression of inserted ILY variant
160528	pTricHis-ILY-HB1lock-L340W	Expression of inserted ILY variant
160529	pTricHis-ILY-HB1lock-N342W	Expression of inserted ILY variant
160530	pTricHis-ILY-HB2lock	Expression of inserted ILY variant
160531	pTricHis-ILY-HB2lock-charge+	Expression of inserted ILY variant
160532	pTricHis-ILY-HB2lock-no charge	Expression of inserted ILY variant
160533	pTricHis-ILY-HB2lock-Y336W	Expression of inserted ILY variant
160534	pTricHis-ILY-HB2lock-N338W	Expression of inserted ILY variant
160535	pTricHis-ILY-HB2lock-L340W	Expression of inserted ILY variant
160536	pTricHis-ILY-HB2lock-N342W	Expression of inserted ILY variant
160537	pTricHis-PLY	Expression of inserted PLY variant
160538	pTricHis-PLY-WT-charge+	Expression of inserted PLY variant
160539	pTricHis-PLY-WT-no charge	Expression of inserted PLY variant