Structural basis for tuning activity and membrane specificity of bacterial cytolysins.
Shah NR, Voisin TB, Parsons ES, Boyd CM, Hoogenboom BW, Bubeck D
Nat Commun. 2020 Nov 16;11(1):5818. doi: 10.1038/s41467-020-19482-6. PubMed Article
Shah NR, Voisin TB, Parsons ES, Boyd CM, Hoogenboom BW, Bubeck D
Nat Commun. 2020 Nov 16;11(1):5818. doi: 10.1038/s41467-020-19482-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160513 | pTricHis-ILY-WT-charge+ | Expression of inserted ILY variant |
| 160514 | pTricHis-ILY-WT-no charge | Expression of inserted ILY variant |
| 160515 | pTricHis-ILY-WT-Y336W | Expression of inserted ILY variant |
| 160516 | pTricHis-ILY-WT-N338W | Expression of inserted ILY variant |
| 160517 | pTricHis-ILY-WT-L340W | Expression of inserted ILY variant |
| 160518 | pTricHis-ILY-WT-N342W | Expression of inserted ILY variant |
| 160519 | pTricHis-ILY-prepore | Expression of inserted ILY variant |
| 160520 | pTricHis-ILY-prepore-L340C | Expression of inserted ILY variant |
| 160521 | pTricHis-ILY-prepore-N342C | Expression of inserted ILY variant |
| 160522 | pTricHis-ILY-prepore-charge+ | Expression of inserted ILY variant |
| 160523 | pTricHis-ILY-HB1lock | Expression of inserted ILY variant |
| 160524 | pTricHis-ILY-HB1lock-charge+ | Expression of inserted ILY variant |
| 160525 | pTricHis-ILY-HB1lock-no charge | Expression of inserted ILY variant |
| 160526 | pTricHis-ILY-HB1lock-Y336W | Expression of inserted ILY variant |
| 160527 | pTricHis-ILY-HB1lock-N338W | Expression of inserted ILY variant |
| 160528 | pTricHis-ILY-HB1lock-L340W | Expression of inserted ILY variant |
| 160529 | pTricHis-ILY-HB1lock-N342W | Expression of inserted ILY variant |
| 160530 | pTricHis-ILY-HB2lock | Expression of inserted ILY variant |
| 160531 | pTricHis-ILY-HB2lock-charge+ | Expression of inserted ILY variant |
| 160532 | pTricHis-ILY-HB2lock-no charge | Expression of inserted ILY variant |
| 160533 | pTricHis-ILY-HB2lock-Y336W | Expression of inserted ILY variant |
| 160534 | pTricHis-ILY-HB2lock-N338W | Expression of inserted ILY variant |
| 160535 | pTricHis-ILY-HB2lock-L340W | Expression of inserted ILY variant |
| 160536 | pTricHis-ILY-HB2lock-N342W | Expression of inserted ILY variant |
| 160537 | pTricHis-PLY | Expression of inserted PLY variant |
| 160538 | pTricHis-PLY-WT-charge+ | Expression of inserted PLY variant |
| 160539 | pTricHis-PLY-WT-no charge | Expression of inserted PLY variant |