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Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi.
Banta AB, Ward RD, Tran JS, Bacon EE, Peters JM
Curr Protoc Microbiol. 2020 Dec;59(1):e130. doi: 10.1002/cpmc.130. PubMed Article

Plasmids from Article

ID Plasmid Purpose
160666pJMP2754Mobile CRISPRi sfGFP 'test', empty sgRNA
160667pJMP2774Mobile CRISPRi sfGFP 'test', gmc6 sgRNA
160668pJMP2782Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs
160669pJMP2820Mobile CRISPRi sfGFP 'test', empty sgRNA
160670pJMP2822Mobile CRISPRi sfGFP 'test', empty sgRNA
160671pJMP2824Mobile CRISPRi sfGFP 'test', empty sgRNA
160672pJMP2832Mobile CRISPRi sfGFP 'test', gmc6 sgRNA
160673pJMP2834Mobile CRISPRi sfGFP 'test', gmc6 sgRNA
160674pJMP2836Mobile CRISPRi sfGFP 'test', gmc6 sgRNA
160675pJMP2844Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs
160676pJMP2846Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs
160677pJMP2849Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Antibodies from Article