TrichoGate: An Improved Vector System for a Large Scale of Functional Analysis of Trichoderma Genes.
Nogueira-Lopez G, Padilla-Arizmendi F, Inwood S, Lyne S, Steyaert JM, Nieto-Jacobo MF, Stewart A, Mendoza-Mendoza A
Front Microbiol. 2019 Dec 10;10:2794. doi: 10.3389/fmicb.2019.02794. eCollection 2019. PubMed Article
Nogueira-Lopez G, Padilla-Arizmendi F, Inwood S, Lyne S, Steyaert JM, Nieto-Jacobo MF, Stewart A, Mendoza-Mendoza A
Front Microbiol. 2019 Dec 10;10:2794. doi: 10.3389/fmicb.2019.02794. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160701 | pTrichoGate-1 | derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites |
| 160702 | pTrichoGate-2 | gpdh promoter from Trichoderma virens bordered by BsaI restriction sites |
| 160703 | pTrichoGate-3 | tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites |
| 160704 | pTrichoGate-4 | Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites |
| 160705 | pTrichoGate-5 | Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites |
| 160707 | pTrichoGate-7 | For N-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites |
| 160708 | pTrichoGate-8 | For N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites |
| 160710 | pTrichoGate-10 | For C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites |
| 160711 | pTrichoGate-11 | For C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites |
| 160713 | pTrichoGate-13 | For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites |
| 160714 | pTrichoGate-14 | For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites |
| 160715 | pTrichoGate-15 | For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites |
| 160716 | pTrichoGate-16 | T-nos Terminator bordered by BsaI restriction sites |
| 160717 | pTrichoGate-17 | TrpC Terminator bordered by BsaI restriction sites |