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Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells.
Ramirez DH, Aonbangkhen C, Wu HY, Naftaly JA, Tang S, O'Meara TR, Woo CM
ACS Chem Biol. 2020 Apr 17;15(4):1059-1066. doi: 10.1021/acschembio.0c00074. Epub 2020 Mar 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
160739(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody
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160740(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody
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160741(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody
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160742(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
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160743(114) pcDNA3.1-GFP-Flag-JunB-EPEAExpresses a GFP tagged JunB with Flag and EPEA tags.
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160744(133) pcDNA3.1-Nup62-Flag-EPEAExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes
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