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Targeting nuclear receptor NR4A1-dependent adipocyte progenitor quiescence promotes metabolic adaptation to obesity.
Zhang Y, Federation AJ, Kim S, O'Keefe JP, Lun M, Xiang D, Brown JD, Steinhauser ML
J Clin Invest. 2018 Nov 1;128(11):4898-4911. doi: 10.1172/JCI98353. Epub 2018 Oct 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
160941MSCV-HA-Nr4a1Generation of retrovirus for the overexpression of Nr4a1
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160942MSCV-HA-Nr4a1-AAGeneration of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)
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160943MSCV-HA-Nr4a1-Zf1Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)
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160944MSCV-HA-Nr4a1-Zf2Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)
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160945lentiCRISPR v2-Nr4a1sgRNA1Guide RNA 1 to generate Nr4a1 knockout by CRISPR
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160946lentiCRISPR v2-Nr4a1sgRNA2Guide RNA 2 to generate Nr4a1 knockout by CRISPR
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160947pMKO.1-shAspmAspm shRNA in pMKO.1 retroviral vector
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160948pMKO.1-shBub1Bub1 shRNA in pMKO.1 retroviral vector
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160949pMKO.1-shBirc5Birc5 shRNA in pMKO.1 retroviral vector
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160953pMKO.1-shCdk1Cdk1 shRNA in pMKO.1 retroviral vector
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160954pMKO.1-shCdc20Cdc20 shRNA in pMKO.1 retroviral vector
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160955pMKO.1-shCdkn3Cdkn3 shRNA in pMKO.1 retroviral vector
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160956pMKO.1-shCenpfCenpf shRNA in pMKO.1 retroviral vector
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160957pMKO.1-shEsco2Esco2 shRNA in pMKO.1 retroviral vector
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160958pMKO.1-shMki67Mki67 shRNA in pMKO.1 retroviral vector
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160959pMKO.1-shPrc1Prc1 shRNA in pMKO.1 retroviral vector
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160960pMKO.1-shSpc25Spc25 shRNA in pMKO.1 retroviral vector
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160961pMKO.1-shNuf2Nuf2 shRNA in pMKO.1 retroviral vector
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160962pMKO.1-shKif11Kif11 shRNA in pMKO.1 retroviral vector
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162791lentiCRISPR v2-Nr4a1 double gRNAsMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPR
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162793pMKO.1-shCcnb1Ccnb1 shRNA in pMKO.1 retroviral vector
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162794pMKO.1-shCcnb2Ccnb2 shRNA in pMKO.1 retroviral vector
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Antibodies from Article