Model-guided design of mammalian genetic programs.
Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, Leonard JN
Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 10.1126/sciadv.abe9375. Print 2021 Feb. PubMed Article
Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, Leonard JN
Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 10.1126/sciadv.abe9375. Print 2021 Feb. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161526 | pJM410 ZF1x6-C mKate2 in TUPV1 | Inducible expression of mKate2 under the ZF1x6-C promoter |
| 161527 | pJM502 ZF1x6-C EYFP in TUPV1 | Inducible expression of EYFP under the ZF1x6-C promoter |
| 161528 | pJM584 ZF2x6-C mKate2 in TUPV1 | Inducible expression of mKate2 under the ZF2x6-C promoter |
| 161529 | pJM585 ZF6x6-C mKate2 in TUPV1 | Inducible expression of mKate2 under the ZF6x6-C promoter |
| 161530 | pJM587 ZF10x6-C EYFP in TUPV2 | Inducible expression of EYFP under the ZF10x6-C promoter |
| 161531 | pJM597 ZF(2/6)x3 mKate2 in TUPV1 | Inducible expression of mKate2 under the ZF(2/6)x3 promoter |
| 161532 | pJB002 CMV 3xFLAG-NLS-DsDed-ZF1 | Constitutive expression ofCMV 3xFLAG-NLS-DsDed-ZF1 under the CMV promoter |
| 161533 | pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter |
| 161534 | pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2 | Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter |
| 161535 | pJM505 EF1α 3xFLAG-NLS-DsRed-Express2-ZF1 in TUPV2 | Constitutive expression of 3xFLAG-NLS-DsRed-Express2-ZF1 under the EF1α promoter |
| 161536 | pJM506 EF1α 3xFLAG-NLS-DsDed-ZF1 in TUPV2 | Constitutive expression of 3xFLAG-NLS-DsDed-ZF1 gene under the EF1α promoter |
| 161537 | pJM507 EF1α 3xFLAG-NLS-DsDed-ZF10 in TUPV2 | Constitutive expression of 3xFLAG-NLS-DsDed-ZF10 under the EF1α promoter |
| 161538 | pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter |
| 161539 | pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4 | Constitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoter |
| 161540 | pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2 | Inducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoter |
| 161541 | pJM574 ZF2x6-C 3xFLAG-NLS-DsDed-ZF1 in TUPV2 | Inducible expression of 3xFLAG-NLS-DsDed-ZF1 under the ZF2x6-C promoter |
| 161543 | pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoter |
| 161544 | pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoter |
| 161545 | pJB001 CMV 3xFLAG-NLS-FKBP-ZF2 | Constitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV promoter |
| 161546 | pJB003 ZF1/2x6-C 3xFLAG-NLS-VP16-FRB | Inducible expression of 3xFLAG-NLS-VP16-FRB under the ZF1/2x6-C promoter |
| 161547 | pPD1122 CMV 3xFLAG-NES-PYL1-VP64 in TUPV3 | Constitutive expression of 3xFLAG-NES-PYL1-VP64 under the CMV promoter |
| 161548 | pKD011 CMV 3xFLAG-NLS-ZF2-ABI1 in TUPV2 | Constitutive expression of 3xFLAG-NLS-ZF2-ABI1 under the CMV promoter |
| 161549 | pJB004 CMV 3xFLAG-NLS-VP16-intN | Constitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoter |
| 161550 | pJB005 CMV 3xFLAG-NLS-intC-ZF2 | Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoter |
| 161551 | pJM516 EF1α intC-ZF1-NLS-HA in TUPV2 | Constitutive expression of intC-ZF1-NLS-HA under the EF1α promoter |
| 161552 | pJM522 EF1α intC-ZF10-NLS-HA in TUPV2 | Constitutive expression of intC-ZF10-NLS-HA under the EF1α promoter |
| 161553 | pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoter |
| 161554 | pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2 | Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter |
| 161555 | pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5 | Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter |
| 161556 | pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoter |
| 161557 | pJM555 EF1α 3xFLAG-NLS-DsDed-intN in TUPV2 | Constitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1α promoter |
| 161558 | pJM571 ZF2x6-C 3xFLAG-NLS-VP64-intN in TUPV2 | Inducible expression of 3xFLAG-NLS-VP64-intN under ZF2x6-C the promoter |
| 161559 | pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2 | Inducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoter |
| 161560 | pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2 | Inducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoter |
| 161561 | pJM575 ZF6x6-C 3xFLAG-NLS-DsDed-ZF10 in TUPV2 | Inducible expression of 3xFLAG-NLS-DsDed-ZF10 under the ZF6x6-C promoter |
| 161562 | pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2 | Inducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoter |
| 161563 | pJM577 ZF6x6-C 3xFLAG-NLS-DsDed-intN in TUPV2 | Inducible expression of 3xFLAG-NLS-DsDed-intN under the ZF6x6-C promoter |
| 161564 | pJM588 EF1α 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA under the EF1α promoter |
| 161565 | pJM589 EF1α intN-ZF10-NLS-HA in TUPV2 | Constitutive expression of intN-ZF10-NLS-HA under the EF1α promoter |
| 161566 | pJM590 EF1α 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA under the EF1α promoter |
| 161567 | pJM591 EF1α 3xFLAG-NLS-VP64-ZF1-intN in TUPV2 | Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intN under the EF1α promoter |
| 161568 | pJM592 EF1α 3xFLAG-NLS-DsDed-ZF10-intN-VP64 in TUPV2 | Constitutive expression of 3xFLAG-NLS-DsDed-ZF10-intN-VP64 under the EF1α promoter |
| 161569 | pJM595 ZF6x6-C intC-ZF1-ZF2-NLS-HA in TUPV2 | Inducible expression of intC-ZF1-ZF2-NLS-HA under the ZF6x6-C promoter |
| 161570 | pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2 | Constitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoter |
| 161571 | pJM612 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 in TUPV3 | Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 under the EF1α promoter |
| 161572 | pJM621 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 in TUPV3 | Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1α promoter |
| 161573 | pJM671 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 in TUPV3 | Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 under the EF1α promoter |