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Engineering synthetic morphogen systems that can program multicellular patterning.
Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
Science. 2020 Oct 16;370(6514):327-331. doi: 10.1126/science.abc0033. PubMed Article

Plasmids from Article

ID Plasmid Purpose
162156pHR_EF1a-->sGFP_PGK-->puroRLentiviral vector for constitutive expression of GFP in mammalian cells
162157pHR_EF1a-->GssmCherry-PNELentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells
162223pHR_EF1a-->GFPligLentiviral vector for constitutive expression of GFP ligand in mammalian cells
162224pHR_EF1a-->myc-mtLaG2Lentiviral vector for constitutive expression of Myc GFP nanobody Lag2 in mammalian cells
162225pHR_EF1a-->HA-mtLaG2Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells
162226pHR_EF1a-->myc-mtC11L34Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells
162227pHR_EF1a-->HA-mtC11L34Lentiviral vector for the expression of HA mtC11L34 in mammalian cells
162228pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFPLentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells
162229pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFPLentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells
162230pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells
162231pHR_TRE3GS-->mCherry_PGK-->BFPLentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cells
162232pHR_TRE3GS-->sGFP-P2A-mCherryLentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells
162233pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFPLentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells
162234pHR_TRE3GS-->sGFP-P2A-BFPLentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells
162235pHR_TRE3GS-->GssGFP-P2A-NLSIFPLentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells
162237pHR_Ef1a-->flagLaM4_Notch_tTALentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells
162238pHR_TRE-->2xNLS_IFP_PGK_PuroRLentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cells

Antibodies from Article