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Characterizing the portability of phage-encoded homologous recombination proteins.
Filsinger GT, Wannier TM, Pedersen FB, Lutz ID, Zhang J, Stork DA, Debnath A, Gozzi K, Kuchwara H, Volf V, Wang S, Rios X, Gregg CJ, Lajoie MJ, Shipman SL, Aach J, Laub MT, Church GM
Nat Chem Biol. 2021 Jan 18. pii: 10.1038/s41589-020-00710-5. doi: 10.1038/s41589-020-00710-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
162572pARC8-LambdaRedBeta-EcSSBArabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.
162576pARC8-LambdaRedBeta-EcSSB-EcMutLE32KArabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

Antibodies from Article