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In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish.
Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, Hall TE
Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
163845pT3TS-BASU-EGFPIn vitro transcription of BASU-EGFP. Parton lab clone JRR
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163846pT3TS-TurboID-EGFPIn vitro transcription of TurboID-EGFP. Parton lab clone JRS
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163847pT3TS-miniTurboID-EGFPIn vitro transcription of miniTurboID-EGFP. Parton lab clone JRT
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163848pT3TS-TurboID-CaaXIn vitro transcription of TurboID-CaaX. Parton lab clone JSU
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163849pT3TS-NLS-TurboIDIn vitro transcription of nls-TurboID. Parton lab clone JST
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163850pT3TS-CD44b-TurboIDIn vitro transcription of CD44b-TurboID. Parton lab clone JTJ
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163851pT3TS-Cavin4b-TurboIDIn vitro transcription of Cavin4b-TurboID. Parton lab clone JTA
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163852acta1-Cavin1a-CloverZebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR
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163853acta1-Cavin4a-CloverZebrafish muscle specific expression of Cavin4a-Clover, Parton lab clone DPV
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163854acta1-Cavin4b-CloverZebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZ
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163855acta1-mRuby2-dGBPZebrafish muscle specific expression of mRuby2-dVHH[GFP]
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163856acta1-mRuby2-VHH[GFP]Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX
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163857bact2-mKate2-P2A-TurboID-dVHH[GFP]Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH
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166565pME-BASU-NSMultisite Gateway middle entry clone encoding BASU. Parton lab clone JRO
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166566pME-TurboID-NSMultisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP
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166567pME-miniTurboMultisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ
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166568pME-mKate2-P2A-TurboID-NSMultisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN
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166569pME-Ruby2-NSMultisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA
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166570p3E-TurboIDMultisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS
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Antibodies from Article