A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance.
Patchsung M, Homchan A, Aphicho K, Suraritdechachai S, Wanitchanon T, Pattama A, Sappakhaw K, Meesawat P, Wongsatit T, Athipanyasilp A, Jantarug K, Athipanyasilp N, Buahom J, Visanpattanasin S, Niljianskul N, Chaiyen P, Tinikul R, Wichukchinda N, Mahasirimongkol S, Sirijatuphat R, Angkasekwinai N, Crone MA, Freemont PS, Joung J, Ladha A, Abudayyeh O, Gootenberg J, Zhang F, Chewapreecha C, Chanarat S, Horthongkham N, Pakotiprapha D, Uttamapinant C
CRISPR J. 2022 Nov 11. doi: 10.1089/crispr.2022.0048. PubMed Article
Patchsung M, Homchan A, Aphicho K, Suraritdechachai S, Wanitchanon T, Pattama A, Sappakhaw K, Meesawat P, Wongsatit T, Athipanyasilp A, Jantarug K, Athipanyasilp N, Buahom J, Visanpattanasin S, Niljianskul N, Chaiyen P, Tinikul R, Wichukchinda N, Mahasirimongkol S, Sirijatuphat R, Angkasekwinai N, Crone MA, Freemont PS, Joung J, Ladha A, Abudayyeh O, Gootenberg J, Zhang F, Chewapreecha C, Chanarat S, Horthongkham N, Pakotiprapha D, Uttamapinant C
CRISPR J. 2022 Nov 11. doi: 10.1089/crispr.2022.0048. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163911 | pET28a-MH6-Bsu | Expresses Bsu large fragment for affinity purification |
| 163912 | pET28a-gp32-H6 | Expresses T4 gp32 for bacterial expression and affinity purification |
| 163913 | pET28a-uvsX-H6 | Expresses uvsX for bacterial expression and affinity purification |
| 163914 | pET28a-MH6-uvsY | Expresses uvsY for bacterial expression and affinity purification |