Hybrid QconCAT-Based Targeted Absolute and Data-Independent Acquisition-Based Label-Free Quantification Enables In-Depth Proteomic Characterization of Wheat Amylase/Trypsin Inhibitor Extracts.
Sielaff M, Curella V, Neerukonda M, Afzal M, El Hassouni K, Distler U, Schuppan D, Longin CFH, Tenzer S
J Proteome Res. 2021 Jan 28. doi: 10.1021/acs.jproteome.0c00752. PubMed Article
Sielaff M, Curella V, Neerukonda M, Afzal M, El Hassouni K, Distler U, Schuppan D, Longin CFH, Tenzer S
J Proteome Res. 2021 Jan 28. doi: 10.1021/acs.jproteome.0c00752. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163957 | pET26b(+)-ATI_QconCAT_1 | Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification. |