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A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae.
Dong C, Jiang L, Xu S, Huang L, Cai J, Lian J, Xu Z
ACS Synth Biol. 2020 Sep 18;9(9):2252-2257. doi: 10.1021/acssynbio.0c00218. Epub 2020 Aug 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
163971pRS415-Cas9-VPRTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast
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163972pRS423-SgHHelper plasmid for cloning gRNAs under the control of the SNR52 promoter.
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163973pRS423-TyrSgHHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter
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163974pRS423-ProSgHHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter
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