Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes.
Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. PubMed Article
Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163976 | pLEX307 RFP | Mammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression) |
| 163977 | pLEX307 ASC | Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays) |
| 163978 | pcDNA3.1 6A NLRP1 CARD-FLAG WT | Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag |
| 163979 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT | Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag |
| 163980 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1386E | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163981 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163982 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163983 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163984 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163985 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163986 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E | Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag |
| 163987 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A | Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag |
| 163988 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A | Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag |
| 163989 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A | Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag |
| 163990 | pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R | Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag |
| 163991 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT | Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging |
| 163992 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 163993 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 163994 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 163996 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 163997 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 163998 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 163999 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging |
| 164000 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging |
| 164001 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging |
| 164002 | pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R | Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging |
| 164003 | pDB.His.MBP.3C NLRP1 UPA-CARD WT | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD |
| 164004 | pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation) |
| 164005 | pDB.His.MBP.3C NLRP1 UPA-CARD M1457A | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation) |
| 164006 | pDB.His.MBP.3C NLRP1 UPA-CARD W1460A | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation) |
| 164007 | pDB.His.MBP.3C NLRP1 UPA-CARD E1461R | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation) |
| 164008 | pDB.His.MBP.3C NLRP1 CARD WT | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD |
| 164009 | pDB.His.MBP.3C NLRP1 CARD G1467C | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C |
| 164010 | pDB.His.MBP.3C NLRP1 UPA WT | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA |
| 164011 | pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT | Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging |
| 164013 | pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E | Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 164016 | pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E | Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 164018 | pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A | Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging |
| 164020 | pcDNA3.1 6A CARD8 CARD-FLAG WT | Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag |
| 164021 | pDB.His.MBP.3C CARD8 UPA-CARD WT | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD |
| 164022 | pDB.His.MBP.3C CARD8 CARD WT | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD |
| 164023 | pDB.His.MBP.3C CARD8 CARD A452C | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications) |
| 164024 | pDB.His.MBP.3C CARD8 CARD A452C R459E | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164025 | pDB.His.MBP.3C CARD8 CARD A452C R464E | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164026 | pDB.His.MBP.3C CARD8 CARD A452C E485R | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164027 | pDB.His.MBP.3C CARD8 CARD A452C E490R | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164028 | pDB.His.MBP.3C CARD8 CARD A452C R495E | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164029 | pDB.His.MBP.3C CARD8 CARD A452C D511K | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164030 | pDB.His.MBP.3C CARD8 CARD A452C Y527A | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications) |
| 164031 | pDB.His.MBP.3C CARD8 UPA-STOP | Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA |
| 164032 | ASC-CASP1 Octamer | Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer |