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Mario Capecchi Lab: Additional gene targeting vector construction plasmids
Nam HS, Capecchi MR
Unpublished

Plasmids from Article

ID Plasmid Purpose  
128845pDTA-5Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
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134322rox Neo* roxrox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
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138412attB Neo* attPattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
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138413PB Neo* PBMinimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
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140235F3 Neo* F3F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
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168769lox2272 Neo* lox2272lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
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176504pBS-2xpA2x SV40 polyA sequences
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176505pBS-3xpA3x SV40 polyA sequences
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176506pBS-4xpA4x SV40 polyA sequences
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178546H2B-sfGFP-T2A-iCrepCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
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182338pMOD4-mT2A-mutated_rpsL-BSD-FTemplate plasmid for PCR amplification of initial recombineering cassette
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182339mT2A-td-sfGFP-FNFRecombineering cassette plasmid for T2A-td-sfGFP-FNF
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182340mT2A-tdTomato-FNFRecombineering cassette plasmid for T2A-tdTomato-FNF
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182341mT2A-sfGFP-linker-iCre-ERT2-FNFRecombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF
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182342mT2A-TagRFP-T-linker-dre-PBD*-FNFRecombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF
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182363miR-124Plasmid with 4x miR-124 target sites
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188230IRES-creERT2-FNFEMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
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Antibodies from Article