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Prime Editing Guide RNA Design Automation Using PINE-CONE.
Standage-Beier K, Tekel SJ, Brafman DA, Wang X
ACS Synth Biol. 2021 Feb 19;10(2):422-427. doi: 10.1021/acssynbio.0c00445. Epub 2021 Jan 19. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
164423pHSG1C3single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
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Recombinant Antibodies from Article