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In vivo glucose imaging in multiple model organisms with an engineered single-wavelength sensor.

Keller JP, Marvin JS, Lacin H, Lemon WC, Shea J, Kim S, Lee RT, Koyama M, Keller PJ, Looger LL
Cell Rep. 2021 Jun 22;35(12):109284. doi: 10.1016/j.celrep.2021.109284. (Link opens in a new window) PubMed (Link opens in a new window) Article

The authors recommend using the updated iGlucoSnFR2 from Marvin et al. (2025) instead of the plasmids listed here.

Plasmids from Article

ID Plasmid Purpose
164510pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.
164513pAAV.GFAP.(Cyto)iGlucoSnFRGreen glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.
164514pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

Antibodies from Article