SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses.
Banerjee AK, Blanco MR, Bruce EA, Honson DD, Chen LM, Chow A, Bhat P, Ollikainen N, Quinodoz SA, Loney C, Thai J, Miller ZD, Lin AE, Schmidt MM, Stewart DG, Goldfarb D, De Lorenzo G, Rihn SJ, Voorhees RM, Botten JW, Majumdar D, Guttman M
Cell. 2020 Nov 25;183(5):1325-1339.e21. doi: 10.1016/j.cell.2020.10.004. Epub 2020 Oct 8. PubMed Article
Banerjee AK, Blanco MR, Bruce EA, Honson DD, Chen LM, Chow A, Bhat P, Ollikainen N, Quinodoz SA, Loney C, Thai J, Miller ZD, Lin AE, Schmidt MM, Stewart DG, Goldfarb D, De Lorenzo G, Rihn SJ, Voorhees RM, Botten JW, Majumdar D, Guttman M
Cell. 2020 Nov 25;183(5):1325-1339.e21. doi: 10.1016/j.cell.2020.10.004. Epub 2020 Oct 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164519 | pB-Halo,IRES-eGFP | All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance) |
| 164520 | pB-Halo,IRES-NGFR | All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance) |
| 164521 | pB-6HIS-TEV-mEGFP,IRES-NGFR | All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance) |
| 164522 | pDONR207 SARS-CoV-2 NSP1 Delta RC | Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A) |
| 164523 | pDONR207 SARS-CoV-2 NSP1 124A/125A | Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A) |