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Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules.
Mateju D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao JA
Cell. 2020 Dec 23;183(7):1801-1812.e13. doi: 10.1016/j.cell.2020.11.010. Epub 2020 Dec 11. PubMed Article

Plasmids from Article

ID Plasmid Purpose
164614pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
164615pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyAExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

Antibodies from Article