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Chase Beisel Lab: Rapidly characterizing CRISPR-Cas13 nucleases using cell-free transcription-translation systems
Unpublished

Plasmids from Article

ID Plasmid Purpose  
164857pLsCas13a-gRNA-NTConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.
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164858pBzCas13b-gRNA-1Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.
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164859pBzCas13b-gRNA-2Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.
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164860pBzCas13b-gRNA-3Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.
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164861pBzCas13b-gRNA-NTConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.
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164862pBzCas13bInducible expression of BzCas13b nuclease in bacteria.
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164863pAcrVIA4Inducible expression of AcrVIA4 in bacteria.
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164864pAcrVIA7Inducible expression of AcrVIA7 in bacteria.
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164865pLsCas13a-gRNA-1Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.
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164866pLsCas13a-gRNA-2Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.
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164867pLsCas13a-gRNA-3Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.
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