Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems.
Wandera KG, Beisel CL
Methods Mol Biol. 2022;2404:135-153. doi: 10.1007/978-1-0716-1851-6_7. PubMed Article
Wandera KG, Beisel CL
Methods Mol Biol. 2022;2404:135-153. doi: 10.1007/978-1-0716-1851-6_7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164857 | pLsCas13a-gRNA-NT | Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria. |
| 164858 | pBzCas13b-gRNA-1 | Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria. |
| 164859 | pBzCas13b-gRNA-2 | Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria. |
| 164860 | pBzCas13b-gRNA-3 | Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria. |
| 164861 | pBzCas13b-gRNA-NT | Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria. |
| 164862 | pBzCas13b | Inducible expression of BzCas13b nuclease in bacteria. |
| 164863 | pAcrVIA4 | Inducible expression of AcrVIA4 in bacteria. |
| 164864 | pAcrVIA7 | Inducible expression of AcrVIA7 in bacteria. |
| 164865 | pLsCas13a-gRNA-1 | Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria. |
| 164866 | pLsCas13a-gRNA-2 | Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria. |
| 164867 | pLsCas13a-gRNA-3 | Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria. |