The loopometer: a quantitative in vivo assay for DNA-looping proteins.
Hao N, Sullivan AE, Shearwin KE, Dodd IB
Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284. PubMed Article
Hao N, Sullivan AE, Shearwin KE, Dodd IB
Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164868 | pAS007 | This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest. |
| 164869 | pID1072 | The pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein. |
| 164901 | pAH121 | The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid. |
| 166036 | AH6112 | Loopometer recipient strain: lacI- Oid+ control |
| 166037 | AH6113 | Loopometer recipient strain: lacI+ Oid- control |
| 166038 | AH6114 | Loopometer recipient strain: lacI+ Oid+ |