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Structural basis of antifolate recognition and transport by PCFT.
Parker JL, Deme JC, Kuteyi G, Wu Z, Huo J, Goldman ID, Owens RJ, Biggin PC, Lea SM, Newstead S
Nature. 2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 10.1038/s41586-021-03579-z. PubMed Article

Plasmids from Article

ID Plasmid Purpose
165414pDDGFP-Leu2d_GgPCFTexpression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.
165415pBXPHM3-PCFT_NBExpression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Antibodies from Article