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Acute depletion of METTL3 implicates N (6)-methyladenosine in alternative intron/exon inclusion in the nascent transcriptome.
Wei G, Almeida M, Pintacuda G, Coker H, Bowness JS, Ule J, Brockdorff N
Genome Res. 2021 Aug;31(8):1395-1408. doi: 10.1101/gr.271635.120. Epub 2021 Jun 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
165420pSpCas9_U6_sgRNA_Mettl3CsgRNA construct for targeting Mettl3 C-terminus
165421pTargeting_Mettl3C_FKBP-VTemplate for knockin FBKP-V into Mettl3 C-terminus

Antibodies from Article