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Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy by promoting YAP phase separation.
Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, Zhang L, Li Y, Yang XP, Sun S
Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 10.1016/j.molcel.2021.01.010.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
166439Pet22b-mEGFPBacterial expression of 6x His tagged mEGFP
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166440Pet22b-mEGFP-YAP-N-IDRBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein
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166441Pet22b-mEGFP-YAP-C-IDRBacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein
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166442Pet22b-mCherry-TEAD4-IDRBacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein
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166443Pet22b-YAPBacterial expression of 6x His tagged YAP
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166444Pet22b-YAPL318EBacterial expression of 6x His tagged YAPL318E
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166445Pet22b-YAP4LEBacterial expression of 6x His tagged YAP4LE
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166446Lenti-Cry2-mCherry- YAP-N-IDRExpresses fusion of CRY2PHR with mCherry and YAP-N-IDR
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166447Lenti-Cry2-mCherry- YAP-C-IDRExpresses fusion of CRY2PHR with mCherry and YAP-C-IDR
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166448Lenti-Cry2-mCherry-coiled coilExpresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain
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166449Lenti-Cry2-mCherry-TEAD4-IDRExpresses fusion of CRY2PHR with mCherry and TEAD4-IDR
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166450pmCherry-TEAD4-YBDExpresses fusion of mCherry and TEAD4-YAP binding domain
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166451pmCherry-TEAD4-IDRExpresses fusion of mCherry and TEAD4-IDR
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166452pmCherry-N-EP300Expresses fusion of mCherry and EP300-N-IDR
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166454pmCherry-C-EP300Expresses fusion of mCherry and EP300-C-IDR
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166455Lenti-40XUAS-LucLuciferase reporter
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166457mEGFP-N1-YAPExpresses fusion of mEGFP and YAP
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166458mEGFP-N1-YAPS127AExpresses fusion of mEGFP and YAPS127A
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166459mEGFP-N1-YAPS127A-deltaCCExpresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)
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166460mEGFP-N1 YAPS127A-deltasIDRExpresses fusion of mEGFP and YAPdeltasIDR (delta360-449)
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166461mEGFP-N1 YAPS127A-deltasTADExpresses fusion of mEGFP and YAPdeltasTAD (delta450-504)
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166462mEGFP-N1 YAPS127A- L308EExpresses fusion of mEGFP and YAPS127A- L308E
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166464mEGFP-N1 YAPS127A- L325EExpresses fusion of mEGFP and YAPS127A- L325E
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166465mEGFP-N1 YAPS127A- L318EExpresses fusion of mEGFP and YAPS127A- L318E
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166466mEGFP-N1 YAPS127A- L308/311/325/318E (4LE)Expresses fusion of mEGFP and YAPS127A- L308/311/325/318E (4LE)
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166467pHAGE-neoEmpty vector to express overexpression cDNA
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166468pHAGE-neo-YAPPlasmid coding for YAP and neomycin resistance in a lentiviral backbone.
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166469pHAGE-neo-YAP-deltaCCPlasmid coding for YAP-deltaCC (delta298-359) and neomycin resistance in a lentiviral backbone.
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166472pUC19-YAP-mEGFPHDR template for tagging of endogenous mouse YAP N-terminus with mEGFP
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166473mEGFP-N1-NF2Expresses fusion of mEGFP and NF2
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166474mEGFP-N1-LATS2Expresses fusion of mEGFP and LATS2
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166475mEGFP-N1-MST2Expresses fusion of mEGFP and MST2
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166476mEGFP-N1-SAV1Expresses fusion of mEGFP and SAV1
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166477mCherry-N1-MOB1Expresses fusion of mCherry and MOB1
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166479PL-CRISPR.EFS.tRFP-gIfngr1-1Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1
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166480PL-CRISPR.EFS.tRFP-gIfngr1-2Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1
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166481Lenti-CRISPR-v2-gYap-1Expresses spCas9 and a gRNA targeting mouse Yap
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166482Lenti-CRISPR-V2-gYap-2Expresses spCas9 and a gRNA targeting mouse Yap
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166485PLKO.1-YAP shRNA2Human YAP RNAi
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166486PLKO.1-Yap shRNAMouse Yap RNAi
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166487PLKO.1-Cd155 shRNA1Mouse Cd155 RNAi
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166488PLKO.1-Cd155 shRNA2Mouse Cd155 RNAi
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Recombinant Antibodies from Article