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ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER.

Weigel AV, Chang CL, Shtengel G, Xu CS, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J, Qiu W, Hess HF, Lippincott-Schwartz J
Cell. 2021 Apr 29;184(9):2412-2429.e16. doi: 10.1016/j.cell.2021.03.035. Epub 2021 Apr 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128155SarI T39N YFPExpression of Sar1 with T39N dominant negative mutation
166892Halo-Sec23Amammalian expression of Sec23A tagged with HaloTag
166893mEmerald-Sec23Amammalian expression of Sec23A tagged with mEmerald
166894mCherry-Sec23Amammalian expression of Sec23A tagged with mCherry
166895Halo-Sec31mammalian expression of Sec31 tagged with HaloTag
166896Halo-p58mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag
166897Sar1b-YFPmammalian expression of Sar1b tagged with YFP
166898Sar1b-H79G-YFPmammalian expression of Sar1b-H79G tagged with YFP
166899Sar1b-mOrange2mammalian expression of Sar1b tagged with mOrange2
166900Sar1b-H79G-mOrange2mammalian expression of Sar1b-H79G tagged with mOrange2
166901Halo-TNFa-RUSHRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo
166902mApple-TNFa-RUSHRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple
166903Halo-GPI-RUSHRUSH cargo: Str-KDEL_IRES_SBP-Halo-GPI
166904mApple-GPI-RUSHRUSH cargo: Str-KDEL_IRES_SBP-mApple-GPI
166905Halo-TfR-RUSHRUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-Halo
166906mApple-TfR-RUSHRUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple
166907Halo-VSVG-RUSHRUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

Antibodies from Article